other:Article Title: Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma
Article Snippet: The cell lines used are as follows: GES-1 (CL-0563, Procell, Wuhan, China), AGS (CL-0022, Procell, Wuhan, China), SGC-7901 (CL-0206, Procell, Wuhan, China), MGC803 (ML-CS-0276, ATCC, VA, USA), MKN-45 (CL-0292, Procell, Wuhan, China), and SNU-1 (CL-0474, Procell, Wuhan, China).
Cell Culture:
Article Title: ADAMTS6 Promotes Angiogenesis and Tumor Growth in Gastric Carcinoma.
Article Snippet: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) protease family involves a critical member ADAMTS6, which is implicated in the pathogenesis of various cancers.. This study explores the function of ADAMTS6 in tumor growth and angiogenesis in gastric carcinoma (GC).. Using the TCGASTAD dataset, key genes closely associated with GC prognosis were screened, and their correlation with the angiogenesis pathway was evaluated.
cDNA Synthesis:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Real-time Polymerase Chain Reaction:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
CCK-8 Assay:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Inverted Microscopy:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Imaging:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Recombinant:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Immunostaining:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Fluorescence:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Microscopy:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Flow Cytometry:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Enzyme-linked Immunosorbent Assay:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
TUNEL Assay:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Luciferase:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Reporter Gene Assay:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Plasmid Preparation:Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.
Modification:Article Title: ADAMTS6 Promotes Angiogenesis and Tumor Growth in Gastric Carcinoma.
Article Snippet: A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) protease family involves a critical member ADAMTS6, which is implicated in the pathogenesis of various cancers.. This study explores the function of ADAMTS6 in tumor growth and angiogenesis in gastric carcinoma (GC).. Using the TCGASTAD dataset, key genes closely associated with GC prognosis were screened, and their correlation with the angiogenesis pathway was evaluated.
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